Translator Disclaimer
1 April 2003 A Chemokine, Interferon (IFN)-γ-Inducible Protein 10 kDa, Is Stimulated by IFN-τ and Recruits Immune Cells in the Ovine Endometrium
Author Affiliations +

Proper distribution of immune cells in the uterus is a prerequisite for successful implantation and subsequent placentation, but biochemical signals that govern such events have not been well characterized. In the present study, the cDNA of a chemokine, interferon (IFN)-γ-inducible protein 10 kDa (IP-10), was identified from a cDNA subtraction study between uterine endometrial tissues from Day 17 pregnant and Day 15 cyclic ewes. The effect of IFN-τ on IP-10 expression and the involvement of IP-10 in the recruitment of immune cells were then investigated. Northern blot analysis revealed that large amounts of IP-10 mRNA were present during conceptus attachment to maternal endometrium and early placentation. IP-10 mRNA was localized to monocytes distributed in the subepithelial stroma of pregnant but not cyclic uteri. This finding was supported by the discovery of IP-10 mRNA expression in monocytes but not in lymphocytes, uterine epithelial cells, or stromal cells. Moreover, the expression of IP-10 mRNA by the monocytes was stimulated by IFN-α, IFN-γ, and IFN-τ in a dose-dependent manner, but the expression of IP-10 mRNA by the endometrial explants was most stimulated by IFN-τ. In a chemotaxis assay, migration of peripheral blood mononuclear cells was stimulated by the addition of IFN-τ stimulated-endometrial culture medium, and the effect was significantly reduced by neutralization with an anti-IP-10 antibody. These results suggest that endometrial IP-10 regulated by conceptus IFN-τ regulates recruitment and/or distribution of immune cells seen in the early pregnant uterus.

Kentaro Nagaoka, Akiharu Sakai, Hisashi Nojima, Yoshihito Suda, Yuichi Yokomizo, Kazuhiko Imakawa, Senkiti Sakai, and Ronald K. Christenson "A Chemokine, Interferon (IFN)-γ-Inducible Protein 10 kDa, Is Stimulated by IFN-τ and Recruits Immune Cells in the Ovine Endometrium," Biology of Reproduction 68(4), 1413-1421, (1 April 2003).
Received: 8 July 2002; Accepted: 1 October 2002; Published: 1 April 2003

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top