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1 July 2003 Abnormal Regulation of DNA Methyltransferase Expression in Cloned Mouse Embryos
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Abstract

Cloning by somatic cell nuclear transfer is inefficient. This is evident in the significant attrition in the number of surviving cloned offspring at virtually all stages of embryonic and fetal development. We find that cloned preimplantation mouse embryos aberrantly express the somatic form of the Dnmt1 DNA (cytosine-5) methyltransferase, the expression of which is normally prevented by a posttranscriptional mechanism. Additionally, the maternal oocyte-derived Dnmt1o isoform undergoes little or none of its expected translocation to embryonic nuclei at the eight-cell stage. Such defects in the regulation of Dnmt1s and Dnmt1o expression and cytoplasmic-nuclear trafficking may prevent clones from completing essential early developmental events. Furthermore, aberrant Dnmt1 localization and expression may contribute to the defects in DNA methylation and the developmental abnormalities seen in cloned mammals.

Young Gie Chung, Sarayu Ratnam, J. Richard Chaillet, and Keith E. Latham "Abnormal Regulation of DNA Methyltransferase Expression in Cloned Mouse Embryos," Biology of Reproduction 69(1), (1 July 2003). https://doi.org/10.1095/biolreprod.102.014076
Received: 6 December 2002; Accepted: 1 February 2003; Published: 1 July 2003
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