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1 July 2003 CPEB2, A Novel Putative Translational Regulator in Mouse Haploid Germ Cells
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Abstract

Translational control of specific mRNAs by cytoplasmic polyadenylation has fundamental roles in gametogenesis. The cytoplasmic polyadenylation element binding (CPEB) protein regulates cytoplasmic polyadenylation of mRNAs as a trans factor in oogenesis and spermatogenesis. The CPEB protein contains two RNA recognition motifs and a Zn-finger structure. Proteins (KIAA0940 and KIAA1673) with similar structures are predicted from the genome database, but nothing is known about their expression and function. Here, we report another novel member of the CPEB protein family, CPEB2. Comparison of the amino acid sequences of CPEB family members suggests that the family can be divided structurally and, perhaps, functionally into two groups: the CPEB group, and the CPEB2-KIAA0940-KIAA1673 group. The CPEB2 maps to mouse chromosome distal 5B and is abundantly expressed in testis. However, it was detected by reverse transcription-polymerase chain reaction in all tissues that we examined. It preferentially binds to poly(U) and localizes to the cytoplasm in transfected HeLa cells. The CPEB2 is expressed postmeiotically in mouse spermatogenesis, suggesting a possible role in translational regulation of stored mRNAs in transcriptionally inactive haploid spermatids.

Yasuyuki Kurihara, Motoyuki Tokuriki, Reiko Myojin, Tamaki Hori, Asato Kuroiwa, Yoichi Matsuda, Takayuki Sakurai, Minoru Kimura, Norman B. Hecht, and Seiichi Uesugi "CPEB2, A Novel Putative Translational Regulator in Mouse Haploid Germ Cells," Biology of Reproduction 69(1), 261-268, (1 July 2003). https://doi.org/10.1095/biolreprod.103.015677
Received: 22 January 2003; Accepted: 1 March 2003; Published: 1 July 2003
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