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1 August 2003 Novel Actin-Like Proteins T-ACTIN 1 and T-ACTIN 2 Are Differentially Expressed in the Cytoplasm and Nucleus of Mouse Haploid Germ Cells
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Abstract

We isolated cDNA clones for the novel actin-like proteins T-ACTIN 1 and T-ACTIN 2, which are expressed specifically in the mouse testis. These clones were from a subtracted cDNA library that was enriched for haploid germ cell-specific cDNAs. The mRNA sizes and deduced molecular masses of t-actin 1/mACTl7b and t-actin 2/mACTl7a were 2.2 kilobases (kb) and 1.8 kb, and Mr 43.1 × 103 and Mr 47.2 × 103, respectively. The two deduced amino acid sequences had 60% homology, and they had approximately 40% homology with other actins. The T-ACTINs contained some of the conserved regions seen in other actins. Although the cellular locations of these two proteins are quite different (T-ACTIN-1 was found in the cytoplasm and T-ACTIN-2 was located in the nucleus), the expression of their proteins and mRNAs is controlled during development and limited during spermiogenesis. In contrast, only T-ACTIN-2 was present in sperm heads and tails. These results suggest that T-ACTINs play important roles in sperm function and in the specific morphogenesis of spermatozoa during spermiogenesis.

Hiromitsu Tanaka, Naoko Iguchi, Carlos Egydio de Carvalho, Yuko Tadokoro, Kentaro Yomogida, and Yoshitake Nishimune "Novel Actin-Like Proteins T-ACTIN 1 and T-ACTIN 2 Are Differentially Expressed in the Cytoplasm and Nucleus of Mouse Haploid Germ Cells," Biology of Reproduction 69(2), 475-482, (1 August 2003). https://doi.org/10.1095/biolreprod.103.015867
Received: 25 January 2003; Accepted: 1 March 2003; Published: 1 August 2003
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