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1 August 2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells
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Abstract

Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>1014-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.

Mito Kanatsu-Shinohara, Narumi Ogonuki, Kimiko Inoue, Hiromi Miki, Atsuo Ogura, Shinya Toyokuni, and Takashi Shinohara "Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells," Biology of Reproduction 69(2), 612-616, (1 August 2003). https://doi.org/10.1095/biolreprod.103.017012
Received: 9 March 2003; Accepted: 1 April 2003; Published: 1 August 2003
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