Translator Disclaimer
1 June 2004 Adenosine Triphosphate Concentration and β-d-Glucuronidase Activity as Indicators of Sea Bass Semen Quality
Author Affiliations +
Abstract

The most common parameters used to evaluate sperm quality are motility rate and duration and fertilization ability. In this study, chemical and biochemical parameters of sea bass (Dicentrarchus labrax) sperm were investigated to find an alternative method for evaluating sperm fertilization ability before and after cryopreservation. The biochemical and chemical analyses were performed with fresh and frozen-thawed sperm and seminal plasma. To cryopreserve sperm, 250-μl straws were used. Fertilization ability was evaluated by inseminating eggs (obtained from hormonally stimulated females) with fresh and cryopreserved sperm. The results revealed a linear relationship (P < 0.05) between semen fertilization capacity and some seminal plasma (β-d-glucuronidase activity, potassium concentration) and sperm (ATP concentration, aspartate aminotransferase activity) parameters. Variations in semen fertilization rate could be best described by two multiple regression models: one including the sperm parameters and another including the seminal plasma parameters. For practical application, the use of simple regression models is of value. Fertilization rate in both fresh and cryopreserved sperm was reliably predicted by determining the ATP concentration or the β-d-glucuronidase activity or both.

Loredana Zilli, Roberta Schiavone, Vincenzo Zonno, Carlo Storelli, and Sebastiano Vilella "Adenosine Triphosphate Concentration and β-d-Glucuronidase Activity as Indicators of Sea Bass Semen Quality," Biology of Reproduction 70(6), 1679-1684, (1 June 2004). https://doi.org/10.1095/biolreprod.103.027177
Received: 31 December 2003; Accepted: 1 January 2004; Published: 1 June 2004
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top