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1 August 2004 Cryopreservation of Porcine Embryos Derived from In Vitro-Matured Oocytes
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Abstract

This study describes a cryopreservation method for porcine in vitro-produced (IVP) embryos using as a model parthenogenetic embryos derived from in vitro-matured (IVM) oocytes. IVP embryos at the expanded blastocyst stage were cryopreserved by vitrification using the minimum volume cooling (MVC) method and exhibited an embryo survival rate of 41.2%. Survival was then significantly improved (83.3%, P < 0.05) by decreasing the amount of cytoplasmic lipid droplets (delipation) prior to vitrification. IVP embryos at the 4-cell stage also survived cryopreservation when vitrified after delipation (survival rate, 36.0%), whereas post-thaw survival of nondelipated embryos was quite low (9.7%). Furthermore, it was demonstrated that porcine IVP morulae can be cryopreserved by vitrification following delipation by a noninvasive method (survival rate, 82.5%). These results clearly confirm that porcine embryos derived from IVM oocytes can be effectively cryopreserved with high embryo survival using the MVC method in conjunction with delipation.

Ritsuko Esaki, Hideto Ueda, Mayuko Kurome, Kazumasa Hirakawa, Ryo Tomii, Hiroki Yoshioka, Hitoshi Ushijima, Masashige Kuwayama, and Hiroshi Nagashima "Cryopreservation of Porcine Embryos Derived from In Vitro-Matured Oocytes," Biology of Reproduction 71(2), 432-437, (1 August 2004). https://doi.org/10.1095/biolreprod.103.026542
Received: 13 December 2003; Accepted: 1 March 2004; Published: 1 August 2004
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