Translator Disclaimer
1 August 2004 Generation of Transgenic Cattle by Lentiviral Gene Transfer into Oocytes
Author Affiliations +
Abstract

The potential benefits of transgenic cattle range from the production of large quantities of pharmaceutically relevant proteins to agricultural improvement. However, the production of transgenic cattle is presently time-consuming and expensive because of the inefficiency of the classical DNA microinjection technique. Here, we report the use of lentiviruses for the efficient generation of transgenic cattle. Initial attempts to produce transgenic cattle by lentiviral infection of preimplantation embryos were not successful. In contrast, infection of bovine oocytes with lentiviral vectors carrying an enhanced green fluorescent protein (eGFP) expression cassette followed by in vitro fertilization resulted in the birth of transgenic calves. Furthermore, all of the calves generated by infection of oocytes were transgenic, and 100% of these animals expressed eGFP as detected by in vivo imaging and Western blotting. In addition, a transgenic calf was produced by infection of fetal fibroblasts followed by nuclear transfer into enucleated oocytes. Taken together, after adjusting lentiviral transgenesis to cattle, unprecedented high transgenesis and expression rates were achieved.

Andreas Hofmann, Valeri Zakhartchenko, Myriam Weppert, Heidi Sebald, Hendrik Wenigerkind, Gottfried Brem, Eckhard Wolf, and Alexander Pfeifer "Generation of Transgenic Cattle by Lentiviral Gene Transfer into Oocytes," Biology of Reproduction 71(2), 405-409, (1 August 2004). https://doi.org/10.1095/biolreprod.104.028472
Received: 17 February 2004; Accepted: 1 March 2004; Published: 1 August 2004
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top