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1 November 2004 Possible Role of Interleukin-1 in the Regulation of Bovine Corpus Luteum Throughout the Luteal Phase
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Abstract

Interleukin-1 (IL-1) is one of the principal cytokines that participate in local regulation of many reproductive functions. The present study was undertaken to determine whether mRNAs for IL-1α, IL-1β, and IL-1 type I receptor (IL-1R) are expressed in bovine corpora lutea (CL), and whether luteal cells respond to treatment with IL-1α and IL-1β during the luteal phase. Bovine CL were classified into five stages (early, Days 2–3; developing, Days 5–6; mid, Days 8–12; late, Days 15–17; and regressed, Days 19–21). IL-1α, IL-1β, and IL-1R mRNAs were detected by reverse transcription-polymerase chain reaction (PCR) in all luteal stages examined. Densitometric analysis of PCR products revealed increases of the mRNA of IL-1α and IL-1R in the CL of the regressed stage (P < 0.05). There was less mRNA for IL-1β in the regressed stage than in the developing and mid stages (P < 0.05). When developing, mid, and late luteal cells were treated with IL-1α (1–30 ng/ml) or IL-1β (1–30 ng/ml) for 24 h, IL-1α and IL-1β dose-dependently increased prostaglandin (PG) F and PGE2 production by the luteal cells of all stages (P < 0.05), indicating the presence of functional IL-1R in bovine CL. However, progesterone synthesis was not affected by either IL-1α or IL-1β treatment. Stimulation with IL-1α and IL-1β decreased the PGE2:PGF ratio in the developing stage (P < 0.05), whereas it increased the ratio in the mid stage (P < 0.05). In the late stage, the ratio of IL-1β-treated cells was greater than that of IL-1α-treated cells (P < 0.05). Overall results indicate that genes for IL-1α and IL-1β are expressed and a functional IL-1R is present in the bovine CL throughout the luteal phase, and suggest that IL-1α and IL-1β have different roles as local modulators to regulate PGF and PGE2 production during the luteal phase.

Ryo Nishimura, Anom Bowolaksono, Tomas J. Acosta, Shuko Murakami, Katarzyna Piotrowska, Dariusz J. Skarzynski, and Kiyoshi Okuda "Possible Role of Interleukin-1 in the Regulation of Bovine Corpus Luteum Throughout the Luteal Phase," Biology of Reproduction 71(5), 1688-1693, (1 November 2004). https://doi.org/10.1095/biolreprod.104.032151
Received: 14 May 2004; Accepted: 1 July 2004; Published: 1 November 2004
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