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1 January 2005 CDC6 Requirement for Spindle Formation During Maturation of Mouse Oocytes
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Abstract

A master regulator of DNA replication, CDC6 also functions in the DNA-replication checkpoint by preventing DNA rereplication. Cyclin-dependent kinases (CDKs) regulate the amount and localization of CDC6 throughout the cell cycle; CDC6 phosphorylation after DNA replication initiation leads to its proteolysis in yeast or translocation to the cytoplasm in mammals. Overexpression of CDC6 during the late S phase prevents entry into the M phase by activating CHEK1 kinase that then inactivates CDK1/cyclin B, which is essential for the G2/M-phase transition. We analyzed the role of CDC6 during resumption of meiosis in mouse oocytes, which are arrested in the first meiotic prophase with low CDK1/cyclin B activity; this is similar to somatic cells at the G2/M-phase border. Overexpression of CDC6 in mouse oocytes does not prevent resumption of meiosis. The RNA interference-mediated knockdown of CDC6, however, reveals a new and unexpected function for CDC6; namely, it is essential for spindle formation in mouse oocytes.

Martin Anger, Paula Stein, and Richard M. Schultz "CDC6 Requirement for Spindle Formation During Maturation of Mouse Oocytes," Biology of Reproduction 72(1), (1 January 2005). https://doi.org/10.1095/biolreprod.104.035451
Received: 18 August 2004; Accepted: 1 September 2004; Published: 1 January 2005
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