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1 March 2005 Testosterone Stimulates Follicle-Stimulating Hormone β Transcription via Activation of Extracellular Signal-Regulated Kinase: Evidence in Rat Pituitary Cells
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Abstract

This study investigated whether estradiol (E2) or testosterone (T) activate extracellular signal-regulated kinase (ERK) and calcium/calmodulin-dependent kinase II (Ca/CaMK II), as indicated by enzyme phosphorylation in rat pituitaries. In vivo studies used adult female rats given E2, T, or empty silastic capsules (vehicle controls). Twenty-four hours later, the rats were given a single pulse of GnRH (300 ng) or BSA-saline (to controls) and killed 5 min later. GnRH stimulated a two- to three-fold rise in activated Ca/CaMK II, and E2 and T had no effect on Ca/CaMK II activation. In contrast, both GnRH and T stimulated threefold increases in ERK activity, with additive effects seen following the combination of GnRH T. E2 had no effect on ERK activity. In αT3 clonal gonadotrope cells, dihydrotestosterone did not activate ERK alone but enhanced and prolonged the ERK responses to GnRH, demonstrating direct effects on the gonadotrope. Thus, the ERK response to GnRH plus androgen was enhanced in both rat pituitary and αT3 cells. In vitro studies with cultured rat pituitary cells examined the effect of GnRH±T in the presence of the mitogen-activated protein (MAP) kinase kinase inhibitor, PD-098059 (PD). Results showed that PD suppressed ERK activational and FSHβ transcriptional responses to T. These findings suggest that one site of T regulation of FSHβ transcription is through the selective stimulation of the ERK pathway.

D. J. Haisenleder, L. L. Burger, K. W. Aylor, A. C. Dalkin, H. E. Walsh, M. A. Shupnik, and J. C. Marshall "Testosterone Stimulates Follicle-Stimulating Hormone β Transcription via Activation of Extracellular Signal-Regulated Kinase: Evidence in Rat Pituitary Cells," Biology of Reproduction 72(3), (1 March 2005). https://doi.org/10.1095/biolreprod.104.035196
Received: 12 August 2004; Accepted: 1 October 2004; Published: 1 March 2005
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