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1 February 2007 Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules
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Abstract

Testicular germ cell transplantation into the seminiferous tubules is at present the only way to induce spermatogenesis from a given source of spermatogonial stem cells. Here we show an alternative method that harnesses the self-organizing ability of testicular somatic cells. The testicular cells of embryonic or neonatal mice or rats and of newborn pigs were dissociated into single cells. Each of them reorganized into a tubular structure following implantation into the subcutis of immunodeficient mice. When mouse germline stem (GS) cells derived from spermatogonial stem cells and expanded in culture were intermingled with testicular cells of rodents, they were integrated in the reconstituted tubules and differentiated beyond meiosis into spermatids. Normal offspring were produced by the microinjection of those spermatids into oocytes. This method could be applicable to various mammalian species and useful for producing functional gametes from GS cells in a xenoectopic environment.

Kaoru Kita, Takeshi Watanabe, Kimito Ohsaka, Hirofumi Hayashi, Yoshinobu Kubota, Yoji Nagashima, Ichiro Aoki, Hideki Taniguchi, Toshiaki Noce, Kimiko Inoue, Hiromi Miki, Narumi Ogonuki, Hiromitsu Tanaka, Atsuo Ogura, and Takehiko Ogawa "Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules," Biology of Reproduction 76(2), 211-217, (1 February 2007). https://doi.org/10.1095/biolreprod.106.056895
Received: 30 August 2006; Accepted: 1 October 2006; Published: 1 February 2007
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