Translator Disclaimer
1 June 2009 The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice
Author Affiliations +
Abstract

In most mammals, the expression of SRY (sex-determining region on the Y chromosome) initiates the development of testes, and thus determines the sex of the individual. However, despite the pivotal role of SRY, its mechanism of action remains elusive. One important missing piece of the puzzle is the identification of genes regulated by SRY. In this study we used chromatin immunoprecipitation to identify direct SRY target genes. Anti-mouse SRY antibody precipitated a region 7.5 kb upstream of the transcriptional start site of cerebellin 4 precursor (Cbln4), which encodes a secreted protein. Cbln4 is expressed in Sertoli cells in the developing gonad, with a profile mimicking that of the testis-determining gene SRY-box containing gene 9 (Sox9). In transgenic XY mouse embryos with reduced Sox9 expression, Cbln4 expression also was reduced, whereas overexpression of Sox9 in XX mice caused an upregulation of Cbln4 expression. Finally, ectopic upregulation of SRY in vivo resulted in ectopic expression of Cbln4. Our findings suggest that both SRY and SOX9 contribute to the male-specific upregulation of Cbln4 in the developing testis, and they identified a direct in vivo target gene of SRY.

Stephen T. Bradford, Ryuji Hiramatsu, Madhavi P. Maddugoda, Pascal Bernard, Marie-Christine Chaboissier, Andrew Sinclair, Andreas Schedl, Vincent Harley, Yoshiakira Kanai, Peter Koopman, and Dagmar Wilhelm "The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice," Biology of Reproduction 80(6), (1 June 2009). https://doi.org/10.1095/biolreprod.108.071480
Received: 25 June 2008; Accepted: 1 February 2009; Published: 1 June 2009
JOURNAL ARTICLE
11 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top