Translator Disclaimer
30 June 2010 Neurotensin and Neurotensin Receptor 1 Expression in Human Myometrium and Uterine Leiomyomas
Author Affiliations +

Leiomyomas or fibroids are the most frequently diagnosed tumors of the female genital tract, and their growth seems to be steroid-hormone dependent by a yet undetermined cellular and molecular mechanism. Sexual hormones induce the secretion of growth factor peptides and the expression of their receptors, stimulating cell proliferation. One of these factors is neurotensin, and increasing evidence suggests that it can promote growth of different cancer cells. Since there are no data on neurotensin expression in normal and tumoral uterine tissue, we have analyzed the expression of NTS and NTSR1 receptor using immunohistochemistry for protein detection, in situ hybridization to detect cells expressing NTS mRNA, and RT-PCR to detect NTSR1 transcript as well as any of the alternative splice variants recently described for this receptor. We found that NTS and NTSR1 are expressed in connective cells of normal myometrium. In leiomyomas, immunoreactivity for NTS and NTSR1 receptor is colocalized in the smooth muscle cells that are also transcribing NTS. Women receiving high doses of steroids for in vitro fertilization showed tumor growth and increased immunoreactivity for neurotensin and NTSR1 receptor. Interestingly, alternative splice variants of NTSR1 receptor were detected only in tumoral tissue. These findings suggest a role of steroid hormones inducing neurotensin expression in leiomyoma smooth muscle cells. In these cells, NTS could act autocrinally through NTSR1 receptor, promoting their proliferation.

Yurena Rodríguez, Teresa A. Almeida, Francisco Valladares, Delia Báez, Francisco Montes de Oca, Candelaria García, Idaira Dorta, Mariano Hernández, Ricardo Reyes, and Aixa R. Bello "Neurotensin and Neurotensin Receptor 1 Expression in Human Myometrium and Uterine Leiomyomas," Biology of Reproduction 83(4), 641-647, (30 June 2010).
Received: 19 March 2010; Accepted: 1 June 2010; Published: 30 June 2010

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top