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22 June 2011 Generation of Viable Embryos and Embryonic Stem Cell-Like Cells from Cultured Primary Follicles in Mice
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Abstract

Primary follicles retrieved from B6CBAF1 prepubertal mice were cultured in a stepwise manner in an alpha-minimum essential medium-based medium to generate viable embryos and embryonic stem cell (ESC)-like cells. A significant increase in follicle growth and oocyte maturation accompanied by increased secretion of 17beta-estradiol and progesterone was achieved by exposing primary follicles to 100 or 200 mIU of follicle-stimulating hormone (FSH) during culture. More oocytes developed into blastocysts following in vitro fertilization (IVF) or parthenogenetic activation after culture with 200 mIU of FSH during the entire culture period than with 100 mIU. Eleven ESC-like cell lines, consisting of four heterozygotic and seven homozygotic phenotypes, were established from 25 trials of primary follicle culture combined with IVF or parthenogenetic activation. In conclusion, primary follicles can potentially yield developmentally competent oocytes, which produce viable embryos and ESC-like cell lines following in vitro manipulation. We suggest a method to utilize immature follicles, which are most abundant in ovaries, to improve reproductive efficiency and for use in regenerative medicine.

Jun Hee Choi, Gil Ah Kim, Jong Heum Park, Gwon Hwa Song, Jun Won Park, Dae Yong Kim, and Jeong Mook Lim "Generation of Viable Embryos and Embryonic Stem Cell-Like Cells from Cultured Primary Follicles in Mice," Biology of Reproduction 85(4), 744-754, (22 June 2011). https://doi.org/10.1095/biolreprod.110.084137
Received: 15 February 2010; Accepted: 1 May 2011; Published: 22 June 2011
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