Responses of growth performance, gut morphology, blood biochemistry, and quality of breast meat to varying lengths of water deprivation were assessed. Ninety broilers were randomly allocated to water deprivation treatments, where water was either supplied ad libitum (0 h) or for 30 min at intervals of 6, 12, 18, and 24 h. Average daily feed intake (ADFI) and gain (ADG) were estimated weekly. Six birds from each treatment were randomly slaughtered. The duodenum, jejunum, and ileum were sectioned to determine gut morphology. Breast meat muscles (pectoralis major) were sampled to determine its physicochemical properties. There were linear decreases in ADG (P < 0.01) and ADFI (P < 0.05), crypt depth (P < 0.01) and crypt width (P < 0.05) in the jejunum, villus height (VH) (P < 0.01) and total mucosa thickness (P < 0.05) in the ileum, crude fat (P < 0.05), b*₀ (yellowness at 0 h), a*₂₄ (redness at 24 h), and L*₂₄ (lightness at 24 h) (P < 0.01), and aspartate aminotransferase enzyme (P < 0.05) with increases in length of water deprivation. Positive quadratic responses of VH in the duodenum (P < 0.01) and jejunum (P < 0.05) were observed after 7.6 and 19.4 h of water deprivation, respectively. Severe water deprivation periods reduced growth performance, but induced positive growth of VH, suggesting that broilers may adapt to water stress. The appropriate length of water deprivation was 7.64 h.