Greter, A. M., AlZahal, O., Duffield, T. F., McBride, W. B., Widowski, T. M. and DeVries, T. J. 2014. Effect of frequency of feed delivery on the feeding behaviour, growth, and rumen temperature of limit-fed dairy heifers. Can. J. Anim. Sci. 94: 11-20. The objective of this experiment was to determine the effect that frequency of provision of feed, provided in a restricted amount, had on feeding and lying behaviour, growth, and rumen temperature of growing dairy heifers. Twenty-four Holstein heifers (178.2±9.3 d of age; mean±SD), divided into six groups of four, were assigned to each of three treatments using a 3×3 Latin square design with 28-d periods (21-d adaptation, 7-d data collection). Treatments were delivery of daily allotment of total mixed ration (fed at 2.0% of body weight): (1) once per day (1×; 0800), (2) twice per day (2×; 0800 and 1600), and (3) four times per day (4×; 0800, 1200, 1600, and 2000). Average daily gain (ADG) was similar across treatments (0.8 kg d^-1). There was a tendency for within-pen variation of ADG (average, per pen, of the absolute difference between individual heifer ADG and pen mean ADG) to be greater with 4× (0.3 kg d^-1) and 2× (0.3 kg d^-1) compared with 1× (0.2 kg d^-1). Both feeding (63.1 vs. 44.2 vs. 51.5 min d^-1) and unrewarded (presence at the feed bunk when no feed was present; 49.5 vs. 27.7 vs. 33.1 min d^-1) time at the feed bunk decreased with increasing frequency of feed delivery (1×, 2×, and 4×, respectively). Frequency of displacements (3.1 displacements d^-1) and number of displacements per unit of feeding time (0.06 displacements min^-1) were similar between treatments. Lying time was similar between treatments (802.5 min d^-1), while there was an increase in the amount of time spent standing without eating as frequency of feed delivery increased (558.9 vs. 590.0 vs. 604.7 min d^-1 for 1×, 2×, and 4×, respectively). Thus, feeding 1× d^-1 may prove beneficial for limit-fed heifers as it increases the time spent feeding, and decreases time spent standing without eating and within-pen variation in ADG.