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13 November 2013 Intestine-specific expression of the β-glucanase in mice
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Guan, L.-Z., Xi, Q.-Y., Sun, Y.-P., Wang, J.-L., Zhou, J.-Y., Shu, G., Jiang, Q.-Y. and Zhang, Y.-L. 2014. Intestine-specific expression of the β-glucanase in mice. Can. J. Anim. Sci. 94: 287-293. The β-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was cloned into a specific expression plasmid (MUC2-GLU-LV). Transgenic mice were prepared by microinjection. Polymerase chain reaction and Southern blot analysis of genomic DNA extracted from the tail tissue of transgenic mice showed that the mice carried the β-glucanase gene. Northern blot analysis indicated that β-glucanase was specifically expressed in the intestine of the transgenic mice. The β-glucanase activity in the intestinal contents was found to be 1.23±0.32 U mL-1. The crude protein, crude fat digestibility of transgenic mice were increased by 9.32 and 5.09% (P<0.05), respectively, compared with that of the non-transgenic mice, while moisture in feces was reduced by 12.16% (P<0.05). These results suggest that the expression of β-glucanase in the intestine of animals offers a promising biological approach to reduce the anti-nutritional effect of β-glucans in feed.

Li-Zeng Guan, Qian-Yun Xi, Yu-Ping Sun, Jing-Lan Wang, Jun-Yun Zhou, Gang Shu, Qing-Yan Jiang, and Yong-Liang Zhang "Intestine-specific expression of the β-glucanase in mice," Canadian Journal of Animal Science 94(2), 287-293, (13 November 2013).
Received: 18 August 2013; Accepted: 1 November 2013; Published: 13 November 2013

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