This experiment was conducted to evaluate the effect of extracted rice bran supplementation in the diet on production performance, feed intake, egg quality, and excreta microflora in laying hens. A total of 288 Hy-line Brown (46 wk old) laying hens were randomly assigned to three different treatments. The three treatments were (1) CON (control), basal diet; (2) T1, basal diet 0.01% extracted rice bran (ERB); and (3) T2, basal diet 0.02% ERB. The trial lasted for 6 wk. Egg quality was checked weekly, whereas egg production was recorded daily throughout the trial period. All data were subjected to the GLM procedures of SAS and differences among treatments were separated by Duncan’s multiple range test with a P < 0.05 indicating a significance. There were eight replicates per treatment with 12 birds per replicate. The results showed that egg production increased linearly during weeks 5, 6, and in cumulative period (P < 0.05). Improved eggshell thickness was found by increasing dietary ERB levels (P < 0.05). The excreta Escherichia coli and Lactobacillus counts decreased and increased respectively by addition of ERB in the diet. In conclusion, dietary inclusion of 0.02% ERB increased egg production, Lactobacillus counts, and decreased E. coli counts in hens.