Abdel-Aal, E. M. and Choo, T.-M. 2014. Differences in compositional properties of a hulless barley cultivar grown in 23 environments in eastern Canada. Can. J. Plant Sci. 94: 807-815. Barley grains hold a promise for several applications in food, feed, energy and malting industries due to their diverse components and functionalities. The current study aimed to investigate compositional and 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging properties of a newly developed hulless barley cultivar, AAC Azimuth. The cultivar was grown at 11 sites in 2010 and 12 sites in 2011 in Ontario and Quebec, Canada. The results showed that most of the nutrients examined in barley varied significantly among locations. In addition several components varied by year such as β-glucan, protein and phenols. There were significant year×location interactions for all of these nutrient components. The application of fungicides increased the content of moisture, but it decreased the content of phenols. The new cultivar contained relatively low amount of dietary fiber, average amount of β-glucan, and high levels of starch and protein, which could improve the feed and energy value. The cultivar also had total phenols and DPPH antioxidant capacity within the range of yellow and color barleys showing its antioxidant potential. The absence of hulls along with reasonable levels of protein, total antioxidant phenols and DPPH antioxidant capacity would promote the cultivar for food use subject to flour characteristics and functionality.