Jiang, Y., Caldwell, C. D. and Falk, K. C. 2014. Camelina seed quality in response to applied nitrogen, genotype and environment. Can. J. Plant Sci. 94: 971-980. Camelina (Camelina sativa L.), Brassicaceae, has great potential for food and industrial use. This study analyzed the seed oil content, oil yield, protein content, protein yield, as well as fatty acid profile relative to varying nitrogen (N) rates and in different genotypes under several environmental conditions. Seed samples were obtained from a 2-yr field study with five environments (site-years), five genotypes, and six N rates. Applied N increased protein content, protein yield, oil yield, and polyunsaturated fatty acids (PUFA), but decreased oil content and monounsaturated fatty acids (MUFA). Saturated fatty acids did not respond consistently to applied N. Lower air temperatures during the reproductive stages increased the total seed oil content, but the fatty acid composition was not affected. The experimental line CDI007 had the highest oil content, oil yield, protein yield, and PUFA, but contained the lowest protein content and MUFA. CDI002 contained the highest protein content and PUFA. CDI005 had the highest amount of MUFA. CDI008 was not considered to be a promising genotype since it had the lowest oil content and highest amount of saturated fatty acids.