Challenges in the emasculation of female parent panicles and limited morphological variation among canary seed (Phalaris canariensis L.) genotypes has resulted in a need for molecular markers to assess hybridity and seed purity. Codominant simple sequence repeat (SSR) markers can be used to assess hybridity and purity instead of a grow-out test. A set of 15 SSR markers was used to assess hybridity of 10 F1-derived F2 families developed from a cross between two glabrous lines: a yellow-seeded C05091 (female parent) and a brown-seeded C00038 (male parent). The two most informative SSR markers, CSM8 and CSM39, could confirm that 9 of 10 families were hybrids due to the presence of a marker allele from the male parent. Three SSR markers, CSM58, CSM85, and CSM102, allowed us to assess the genetic identity of four experimental canary seed lines: C08019, C08046, C09052, and C10022. The three markers also identified the physical admixture in 1 of 16 batches of seeds.
How to translate text using browser tools
23 February 2017
Utilization of microsatellite markers to assess hybridity and genetic identity of canary seed (Phalaris canariensis L.) genotypes
U. Kannan,
A.F. Altaher,
M. Båga,
P. Hucl,
R.N. Chibbar
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
Alpiste roseau
Canary seed
genetic identity and hybridity
identité génétique et hybridité
simple sequence repeats
SSR