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23 February 2017 Utilization of microsatellite markers to assess hybridity and genetic identity of canary seed (Phalaris canariensis L.) genotypes
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Abstract

Challenges in the emasculation of female parent panicles and limited morphological variation among canary seed (Phalaris canariensis L.) genotypes has resulted in a need for molecular markers to assess hybridity and seed purity. Codominant simple sequence repeat (SSR) markers can be used to assess hybridity and purity instead of a grow-out test. A set of 15 SSR markers was used to assess hybridity of 10 F1-derived F2 families developed from a cross between two glabrous lines: a yellow-seeded C05091 (female parent) and a brown-seeded C00038 (male parent). The two most informative SSR markers, CSM8 and CSM39, could confirm that 9 of 10 families were hybrids due to the presence of a marker allele from the male parent. Three SSR markers, CSM58, CSM85, and CSM102, allowed us to assess the genetic identity of four experimental canary seed lines: C08019, C08046, C09052, and C10022. The three markers also identified the physical admixture in 1 of 16 batches of seeds.

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U. Kannan, A.F. Altaher, M. Båga, P. Hucl, and R.N. Chibbar "Utilization of microsatellite markers to assess hybridity and genetic identity of canary seed (Phalaris canariensis L.) genotypes," Canadian Journal of Plant Science 97(5), (23 February 2017). https://doi.org/10.1139/cjps-2016-0129
Received: 21 April 2016; Accepted: 1 February 2017; Published: 23 February 2017
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