Kumaragamage, D., Flaten, D., Akinremi, O. O., Sawka, C. and Zvomuya, F. 2011. Soil test phosphorus changes and phosphorus runoff losses in incubated soils treated with livestock manures and synthetic fertilizer. Can. J. Soil Sci. 91: 375-384. Source of phosphorus (P) and soil properties influence changes in soil test P (STP) concentrations and P runoff losses in manured and fertilized soils. We compared STP changes and P runoff losses in two soils, a clay loam and a sand, that were either unamended (control), or amended with liquid swine manure (LSM), solid cattle manure (SCM), or monoammonium phosphate (MAP) and incubated for 6 wk. Soil subsamples after incubation were analyzed for STP using Olsen (OP), Modified Kelowna (KP), Mehlich 3 (M3P) and water extraction (WEP) methods. We collected runoff from incubated soils for 60 min under a rainfall simulator, and analyzed for dissolved reactive P (DRP). Magnitude of STP increase in amended soils was greater in sand (19-48%) than in clay loam (7-37%). Increases in STP and DRP runoff concentrations in amended soils generally followed the order; MAP>LSM>SCM. Olsen P, KP and M3P were more accurate than WEP for predicting runoff DRP concentrations and loads, accounting for 43-49% of the variation in P concentrations for the first 30 min of runoff. Olsen P, the currently used STP method for environmental P regulation in Manitoba is sufficiently robust to predict runoff P losses from manure and fertilizer amended soils.