The Jamaican Boa Chilabothrus subflavus (Boidae) is a Jamaican-protected species whose numbers and distribution have declined during the last century. In order to protect Jamaican Boas, translocation has been recommended as a conservation strategy. From December 2010 to November 2012 a short-distance translocation (SDT) of seven resident female Jamaican Boas in Windsor, Trelawny Parish, Jamaica was conducted to assess the effects of SDT on the survivability, home range size and microhabitat use of translocated individuals. A subset of seven resident (non-translocated) Jamaican Boas radiotracked in Windsor from November 2008 to June 2009 was used for comparison to SDT snake data. No mortalities or significant differences in home range size were recorded for SDT as compared to resident female Jamaican Boas in Windsor. Visual detectability was higher for resident as compared to SDT snakes, however, both groups utilized arboreal microhabitats in greater proportion to terrestrial locations. Both groups were found in areas characterized by tall, canopy layer trees with vines and epiphytes, but SDT boas utilized larger trees with greater epiphyte densities than resident snakes. Results suggest short-distance translocation has potential as a management strategy for the conservation of Jamaican Boa populations in Jamaica.