A transplantation technique for transferring adult worms of Ascaridia galli to parasite-naive recipient chickens is described. The aim was to find a less traumatic transplantation technique than surgical transplantation, as well as a technique that would avoid worm injuries due to worm passage through the chicken proventriculus and gizzard. Thirty experimental growers were divided into 3 groups. Group 1 animals were fasted for 24 hr before transplantation, and group 2 received an antiperistaltic treatment before and after the transplantation. Animals in group 3 received no special treatment. All animals were fasted for 2 hr before anesthesia and pretreated with atropine. The chickens were anesthetized, and each received 2 male and 2 female worms of Ascarid. galli by cloacal intubation. The tube was inserted and the worms flushed out of the tube as anteriorly as possible in the rectum. Fecal samples were examined during the experimental period to monitor the eventual parasite egg excretion. Two weeks after transplantation the birds were slaughtered and the intestines examined for adult Ascarid. galli. Infection was established in 20% of the animals belonging to groups 1 and 2, and in 60% of the animals in group 3. This transplantation technique enables direct establishment of infections of adult Ascarid. galli in chickens, although the exact worm sex composition of the infection is still unpredictable because of expulsion of worms after transplantation. The method is less traumatic to the experimental animal than are surgical techniques.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.