Prevalence of intestinal parasites was assessed in 1,370 children in Khan Younis Governorate. The age of the children ranged from 6 to 11 yr. Stool samples were examined by direct smear, flotation, and sedimentation techniques. General prevalence of intestinal parasites was 32.4%. Ascaris lumbricoides was found to be the most common parasite (12.8%), followed by Giardia lamblia (8.0%), Entamoeba histolytica (7.0%), Entamoeba coli (3.6%), Trichuris trichiura (1.6%), and Hymenolepis nana (1.0%). Enterobius vermicularis was detected with the scotch tape preparation. Of the examined children, 20.9% were positive for E. vermicularis.