A survey, conducted during May 1998, 1999, and 2006, involving 174 procambarid crayfish from 13 localities within the states of Georgia, Alabama, and Mississippi, U.S.A., indicated that adult Alloglossidium dolandi infected the antennal glands of 5 new host species: Procambarus acutissimus, Procambarus acutus, Procambarus howellae, Procambarus paeninsulanus, and Procambarus troglodytes. Infected crayfish were taken from roadside ditches, streams, backwaters of streams, and a roadside flood pool adjacent to a river. Geographic distribution of the parasite formed a discontinuous belt extending westward from southeastern Georgia, through southern Alabama, and into eastern Mississippi, while transiting each crayfish host species' reported range. Prevalence of infection was 0% at 3 localities but ranged from 21.4% to 92.3% at 10 other localities.