The piscicolid leech, Limnotrachelobdella okae (Moore, 1924), was found to be parasitic on Japanese seaperch, Lateolabrax japonicus (Lateolabracidae), from Tokyo Bay and on kusafugu, Takifugu niphobles (Tetraodontidae), from the Sea of Japan in Yamagata, Japan. This species was also collected from bastard halibut, Paralichthys olivaceus (Paralichthyidae), and Japanese pufferfish, Takifugu rubripes (Tetraodontidae), cultured in the Seto Island Sea, Okayama, Japan. These 4 fish species are new hosts for L. okae. Based on a wide range of fish hosts of different taxonomic groups (11 species in 7 families from 6 orders) including the present 4 new hosts, L. okae apparently shows no strict host specificity.