Twenty eastern pipistrelles (Perimyotis subflavus) were collected in November 2009 and March 2010 from an abandoned mine in Polk County, Arkansas, U.S.A., and examined for helminths. Fifteen (75%) were infected with 1 or more lecithodendriid trematodes, including 2 (10%) with Acanthatrium eptesici, 13 (65%) with Acanthatrium pipistrelli, 5 (25%) with Ochoterenatrema breckenridgei, and 2 (10%) with Paralecithodendrium naviculum; 7 (35%) were multiply infected with 2 trematode species. We document a new host record for P. naviculum, and A. eptesici, O. breckenridgei, and P. naviculum are reported from Arkansas for the first time. We also include a summation of the endoparasites of Perimyotis subflavus.