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20 September 2012 Identification of Polymorphic Loci in Ambystoma annulatum and Review of Cross-species Microsatellite Use in the Genus Ambystoma
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Abstract

We screened 74 published microsatellite primers in Ambystoma annulatum, a species for which no microsatellite markers have been developed. Overall, we had a moderate success rate, identifying 11 polymorphic microsatellites previously developed in four different species of Ambystoma. We also conducted a review of the literature, collecting all published cross-species applications of microsatellite markers within the genus Ambystoma. From this, we identified 20 loci that have amplified in three or more species. Our synthesis of microsatellite use within the genus Ambystoma should prove valuable to future molecular research, especially in species without developed markers and for studies in species that may already have microsatellites, but are being conducted far from the region where individuals were collected for original development of species-specific loci.

2012 by the American Society of Ichthyologists and Herpetologists
William E. Peterman, Grant M. Connette, Brett N. Spatola, Lori S. Eggert, and Raymond D. Semlitsch "Identification of Polymorphic Loci in Ambystoma annulatum and Review of Cross-species Microsatellite Use in the Genus Ambystoma," Copeia 2012(3), 570-577, (20 September 2012). https://doi.org/10.1643/CH-11-001
Received: 3 January 2011; Accepted: 1 March 2012; Published: 20 September 2012
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