Translator Disclaimer
1 June 2016 Decision-Making for the Detection of Amatoxin Poisoning: A Comparative Study of Standard Analytical Methods
Author Affiliations +
Abstract

Amatoxin-containing mushroom poisonings are recorded worldwide and the frequency increases due to confusion with other macrofungi. Affected regions are characterized by important disparities in relation to available technological equipment for analytical identification of amatoxins. In this context the present study was designed to define advantages and disadvantages of the most accessible standard analytical methods for amatoxin detection. Several methods were compared: (1) a commercialized immunoassay kit, (2) standard high-performance liquid chromatography (HPLC) and (3) high-performance thin layer chromatography (HP-TLC). For each method, linearity, limit of detection (LOD), limit of quantification (LOQ) and recovery were determined. Six macrofungi were analysed using these compared methods, three known to contain amatoxins: Amanita phalloides, Amanita virosa, Lepiota josserandii, and three free-amatoxin containing macrofungi: Amanita muscaria, Macrolepiota procera and Omphalotus olearius. Our results will allow for a choice of method with full knowledge of advantages and disadvantages of each technique as a function of local technological possibilities when facing suspected poisoning due to amatoxin-containing mushrooms. The final aim is to be able to reach faster and effective diagnosis in order to save a patient's life.

© 2016 Adac. Tous droits réservés
Sylvie Morel, Françoise Fons, Sylvie Rapior, Virginie Dubois, Manon Vitou, Karine Portet, Jean-Christophe Dore, and Patrick Poucheret "Decision-Making for the Detection of Amatoxin Poisoning: A Comparative Study of Standard Analytical Methods," Cryptogamie, Mycologie 37(2), 217-239, (1 June 2016). https://doi.org/10.7872/crym/v37.iss2.2016.217
Published: 1 June 2016
JOURNAL ARTICLE
23 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top