Translator Disclaimer
1 November 2012 Development of Microsatellite Markers for Cynops ensicauda (Amphibia: Caudata) by Using Next-Generation Sequencing Technology
Author Affiliations +
Abstract

We isolated and characterized seven microsatellite markers including di-, tri-, and tetra-nucleotide repeats for Cynops ensicauda, which is a near-threatened species and endemic to the Central Ryukyus, Japan, using next-generation sequencing. Six out of the seven loci that showed polymorphism in Cynops ensicauda popei on Okinawajima Island were also amplified in C. e. ensicauda on Amamioshima Island. Expected heterozygosities of these markers were 0.00–0.738 (mean, 0.429) and 0.00–0.791 (mean, 0.459) in C. e. popei and C. e. ensicauda, respectively. These markers will be useful for genetic studies, such as evaluating the genetic diversity within and between populations of this species.

© 2012 by The Herpetological Society of Japan
Koji Mochida, Eiji Inoue, Kazuki Kurita, Azusa Hayano, and Miho Inoue-Murayama "Development of Microsatellite Markers for Cynops ensicauda (Amphibia: Caudata) by Using Next-Generation Sequencing Technology," Current Herpetology 31(2), (1 November 2012). https://doi.org/10.5358/hsj.31.117
Accepted: 1 August 2012; Published: 1 November 2012
JOURNAL ARTICLE
4 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top