Reproduction was studied in an invasive population of Anolis carolinensis in the Hawaiian Islands, USA. Timing of events in the reproductive cycle was similar between A. carolinensis populations in Hawaii and native populations of the species in the southeastern United States. In Hawaii, males of A. carolinensis undergo a prolonged period of spermiogenesis (sperm formation) starting in November (n=1) and December (n=1) and continuing into August. Gravid A. carolinensis females in Hawaii (n=40) produce one egg in continuous succession from March into August. Reproductive activity in A. carolinensis in Hawaii ceased prior to the colder, wetter, winter months.