Cloning and sequence characterization of four important genes (actin, ultra spiracle proteins, tyrosine hydroxylase and vATPase B) from Plutella xylostella are compared with other species of Lepidoptera. Sequence analyses showed variation of the genes within Lepidoptera and the number of parsimony informative sites observed in nucleotide sequences was 151, 426, 502 and 103 for actin, ultra spiracle proteins, tyrosine hydroxylase and vATPase B respectively. Phylogenetic analysis indicated that actin and vATPase B are highly conserved across Lepidoptera. This analysis is useful in understanding the genetic relationship among various Lepidopteran insect and thereby providing insight into the genetic structure. This can establish a critical basis for analysis of biological functions in insects using novel approaches such as RNA interference (RNAi).
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.