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1 February 2006 Assessing Nontarget Impacts of Bt Corn Resistant to Corn Rootworms: Tier-1 Testing with Larvae of Poecilus chalcites (Coleoptera: Carabidae)
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Abstract

A dietary exposure bioassay with larvae of the ground beetle, Poecilus chalcites (Say) (Coleoptera: Carabidae), was developed to assess potential nontarget impacts of corn event MON 863 expressing the Cry3Bb1 protein for control of corn rootworms, Diabrotica spp. (Coleoptera: Chrysomelidae). The assay involved 28 d of continuous exposure of P. chalcites larvae to an artificial diet treated with a maximum hazard dose (930 μg/g of diet) of the Cry3Bb1 protein. Results from this study showed that the Cry3Bb1 protein at a concentration of 930 μg/g of diet had no adverse effect on the survival, development, and growth (biomass) of P. chalcites larvae. Furthermore, statistical power analysis indicated that at levels of 80% power and 5% type I error rate, the study design would have been able to detect a minimum 14 (at day 7) to 19% (at days 14, 21, and 28) reduction in survival and a 21 (at day 14) to 18% (at day 28) reduction in biomass of test larvae relative to the negative buffer control groups. Based on the maximum level (93 μg/g) of the Cry3Bb1 protein expressed in MON 863 corn tissues including leaves, roots, and pollen, findings from this study indicate that corn hybrids containing the MON 863 event have a minimum 10 times safety factor for larvae of P. chalcites, and thus pose little risk to this nontarget beneficial insect.

Jian J. Duan, Mark S. Paradise, Jonathan G. Lundgren, Jeffrey T. Bookout, Changjian Jiang, and Robert N. Wiedenmann "Assessing Nontarget Impacts of Bt Corn Resistant to Corn Rootworms: Tier-1 Testing with Larvae of Poecilus chalcites (Coleoptera: Carabidae)," Environmental Entomology 35(1), 135-142, (1 February 2006). https://doi.org/10.1603/0046-225X-35.1.135
Received: 24 July 2005; Accepted: 1 October 2005; Published: 1 February 2006
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