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1 February 2011 Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera)
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Abstract

Hemocyanins are large oligomeric respiratory proteins found in many arthropods and mollusks. The overall expression of hemocyanin mRNA, revealed by studies on Plecoptera hemocyanin sequencing, has raised the question of whether the protein is expressed or not. In fact, the presence of expressed hemocyanin has only been reported in the literature for one species, Perla marginata (Panzer, 1799). In this paper, we report the presence of hemocyanin and hexamerin proteins in Dinocras cephalotes (Curtis, 1827), a species closely related to P. marginata. To assess the presence of hemocyanin, we used a reproducible and highly sensitive method based on liquid chromatography tandem mass spectrometry. We conclude that regardless of its putative function (respiratory, immune defense, storage protein), the hemocyanin is actually expressed in species in which its mRNA is present.

© 2011 Entomological Society of America
V. Amore, M. A. Puig García, A. M. Timperio, G. Egidi, N. Ubero-Pascal, and R. Fochetti "Comparative Proteomic Analysis of Hemocyanins in Dinocras cephalotes and Perla marginata (Plecoptera)," Environmental Entomology 40(1), (1 February 2011). https://doi.org/10.1603/EN10088
Received: 12 April 2010; Accepted: 1 October 2010; Published: 1 February 2011
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