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1 December 2013 Molecular Characterization and Analysis of Bacterial Diversity in Aleurocanthus woglumi (Hemiptera: Aleyrodidae)
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Abstract

Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae), commonly referred to as citrus blackfly, is a sap-sucking hemipteran insect. Although polyphagous, citrus is its most preferred host plant. Samples of this insect were collected from Murraya koenigii (L.). The cytochrome c oxidase subunit I gene (mtCO1)-based analysis by sequencing helped in molecular identification of the insect. Phylogenetic analysis of cytB-nd1-LrDNA showed the coevolution of A. woglumi with its primary bacterial symbiont Portiera. Sequencing a 16S rDNA library from insect DNA revealed three bacterial phylotypes, namely, Portiera, Wolbachia, and Erwinia chrysanthemi. Further, we used fluorescence in situ hybridization to visualize the endosymbionts in a whole mount of A. woglumi. Culturable bacteria were obtained on different media and were classified on the basis of 16S rDNA. In total, 30 bacterial phylotypes belonging to 14 different genera, namely, Bacillus, Kocuria, Micrococcus, Staphylococcus, Paenibacillus, Rhodococcus, Rummellibacillus, Arthrobacter, Curtobacterium, Psychrobacillus, Listeria, Brevibacillus, Bhargavae, and Pantoea, were isolated by culturable methods.

© 2013 Entomological Society of America
Neeti Pandey, Ambika Singh, Vipin Singh Rana, and R. Rajagopal "Molecular Characterization and Analysis of Bacterial Diversity in Aleurocanthus woglumi (Hemiptera: Aleyrodidae)," Environmental Entomology 42(6), 1257-1264, (1 December 2013). https://doi.org/10.1603/EN13110
Received: 22 April 2013; Accepted: 29 August 2013; Published: 1 December 2013
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