From 71 collections 48 species representing 29 genera of lichens were found during a one-day foray in the Hoffman Trail area of Theodore Roosevelt Natural Area, North Carolina in December 2006. All but one were found on bark, with nearly equal representation in the midbole and canopy layers of the forest; only one species was found growing on the forest floor. This checklist supplements that from a survey of the Natural Area conducted during the 12^th Tuckerman Workshop in 2003, which focused on the Theodore Roosevelt Nature Trail area. Twenty-seven taxa reported here were not encountered in the 2003 survey and thus represent new additions to the flora. Although not encountered during the survey, a population of the rare Teloschistes flavicans (lichenized Ascomycota: Teloschistiaceae) is reported from the Natural Area by park personnel.