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1 December 2005 THE EFFECT OF INTRASPECIFIC SAMPLE SIZE ON TYPE I AND TYPE II ERROR RATES IN COMPARATIVE STUDIES
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Abstract

Comparative studies have increased greatly in number in recent years due to advances in statistical and phylogenetic methodologies. For these studies, a trade-off often exists between the number of species that can be included in any given study and the number of individuals examined per species. Here, we describe a simple simulation study examining the effect of intraspecific sample size on statistical error in comparative studies. We find that ignoring measurement error has no effect on type I error of nonphylogenetic analyses, but can lead to increased type I error under some circumstances when using independent contrasts. We suggest using ANOVA to evaluate the relative amounts of within- and between-species variation when considering a phylogenetic comparative study. If within-species variance is particularly large and intraspecific sample sizes small, then either larger sample sizes or comparative methods that account for measurement error are necessary.

Luke J. Harmon and Jonathan B. Losos "THE EFFECT OF INTRASPECIFIC SAMPLE SIZE ON TYPE I AND TYPE II ERROR RATES IN COMPARATIVE STUDIES," Evolution 59(12), 2705-2710, (1 December 2005). https://doi.org/10.1554/05-224.1
Received: 22 April 2005; Accepted: 29 September 2005; Published: 1 December 2005
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