The Sterile Insect Technique is an important component of area-wide programs to control invading or established populations of pestiferous tephritids. The sterile insect technique involves the release of large numbers of mass-reared, sterilized males to achieve sterile male × wild female matings, which yield infertile eggs and thus suppress the pest population. The development of male-only strains (also termed genetic sexing strains) has resulted in more effective control of wild populations than standard bisexual releases. A genetic sexing strain based on sex-linked pupal color exists for Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae), an important agricultural pest worldwide, but how this strain might perform in a sterile insect technique program has not been thoroughly investigated. As documented for several tephritid species, artificial selection imposed via mass-rearing, particularly over long periods, may have negative effects on various biological parameters of the released flies, including flight ability and dispersion, life span, and mating competitiveness. The goal of the present study was to compare lure responsiveness, dispersal, and survival between males from genetic sexing strains and wild strains of Z. cucurbitae. Our results indicate that males of the 2 strains differed significantly in dispersal ability, but not in lure attraction or survival ability. The potential usefulness of the genetic sexing strains in sterile insect technique programs for control of Z. cucurbitae is assessed based on these findings.