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1 September 2012 Possible Residual Effect of Pesticide on Bactrocera oleae Rossı (Dıptera:Tephrıtıdae) Adults
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The olive fruit fly, Bactrocera oleae Rossi, is the main pest of olive orchards in the Mediterranean Basin (Economopoulos 2002). The larvae are monophagous (Weens & Nation 2003) and compelete their development in the mesocarp of the olive fruit. Females oviposit eggs inside the fruit and decreasing the value of table fruit. Olive fruit fly larvae cause important damage to table olives, and damage oil production by increasing acidity of olive oil. Small scale rearing on artificial diets was reviewed by Silva (1970), Hagen (1966), Hagen et al. (1963), Mittler & Tsitsipis (1973) and Gene (2008), and on host olive fruits by Tzanakakis (1971) and Gene & Nation (2008). Olive flies reared on an artificial diet differ from wild flies in certain aspects of their physiology, such as Allele frequencies (Economopolos 1980; Loukas et al. 1985; Cosmidis et al. 2002). The traditional control of this pest has been based on organophosphate insecticides for years in Turkey and many other countries. However intensive use of insecticides has lead to the development of acetylcholinesterase (AChE) enzyme resistance rsulting in 2 resistance mutations (I199V and G488S) (Vontas et al. 2002). Skavdis et al. (2009) found resistance-associated point mutations of organophosphate-insensitive acetylcholinesterase (AChE) in wild populations of olive fruit fly in Çanakkale, Turkey. The sterile insect technique (SIT) is the most promising approach for fruit fly integrated management (Enkerlin & Mumford 1997; Hendrichs et al. 2002). A primary requirement for successful olive fruit flie SIT is mass rearing flies on a larval diet of high efficient, and low cost larval diet. It is also necessary that the larvae should have similar characteristics as the wild populations with comparable variability, vigor and behavior. The primary aim of my work has been to obtain information on laboratory rearing and physiology of reared flies. In this paper, I report an observation of possible pesticide residual effect on olive fruit fly adults.

Fig. 1.

The appearance of paralyzed olive fruit fly females (A, B and C).

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An olive fruit fly colony was established from naturally infested fruits. Flies have been reared on an artificial diet in the laboratory since 2006. The larval diet is based on soy hydrolysate, yeast and sugar (Tsitsipis & Kontos 1983). Adult flies were kept in fine mesh screen cages (30 × 30 × 30 cm) and provided with a liquid diet containing water, sugar and brewer's hydrolyzed yeast (Tzanakakis 1971) with access also to solid diet containing hydrolyzed brewer's yeast, sugar, dried egg yolk powder and streptomycin sulfate (Tsitsipis & Kontos 1983). Water was supplied by dental wicks in adult cages.

Green olive fruits, as a natural diet, were obtained from the local market. Adults (n = 200, 120 ♀ :80 ♂) that had been laboratory reared were allowed to oviposit in 20–30 unripe locally grown ‘Gemlik’ variety table olives. After 4–5 h, some adults were observed lying motionless at the bottom of the cages. They were unable to fly or walk. Females especially appeared to be paralyzed. Flies had the third pair of legs extended posteriorly (Fig. 1). Flies were still alive but they could not move their legs or wings. A total of 129 flies (some male and some female) were picked up gently with soft forceps and placed in a petri dish. A camel hair brush was wetted with distilled H2O and the body parts (head, thorax, legs, wings, and abdomen) were gently washed twice under an Olympus SZX9 streozoom microscope, and then the flies placed back in the cage. The cage was monitored after 24 h. I found that 70 adult males were able to recover from the residual effect of the pesticide on the surface of the olive fruit. The remaining 59 adults - all females - could not recover. They were dead on the following d. Typical behavior is that females usually walk on the fruit with the mouthparts tapping the fruit surface to select fruit in which to oviposit (Gene & Nation 2008). This behavior may cause females to accumulate more pesticide residue than males. Some of the adults originally placed in the cage (61♀ :80 ♂) survived 18.3 ± 4.7 d in the laboratory. Parafin domes were placed in the cage as typically used in the laboratory adult colony for oviposition, but the females laid no eggs during their lifetimes.

It is important to recognize that the residue on the surface of the olive fruit dramatically affected the olive fruit fly adults. Further studies are needed to understand the residual effect of table olives and olive oils on the environment and on human health.

I thank to The Turkish Academy of Sciences (TÜBA-GEBİP 2009) for financial support.

SUMMARY

Adult olive fruit flies appeared to be affected by residual insectide deposits on the surface of the olive fruit. Females initially knocked down did not recover, and all died, but knocked down males recovered by the next day after washing their bodies with water. In future experiments with olives of unknown history, the researcher is advised to wash the fruit thoroughly before offering then to flies.

REFERENCES CITED

1.

N. Cosmidis , M. Luokas V. Peppa , G. Goulielmos , and E. Zouros 2002. Effect of acetone feeding on alcohole dehydrogenase activity in the olive fruit fly, Bactrocera oleae. Heredity 89: 453–459 pp. Google Scholar

2.

A. P. Economopoulos 1980. SIRM against the olive fruit fly: Differences between wild and lab-reared (normal or sterilized) insects, pp. 17–26 In J. Coyama [ed.], Proc. XVI I Int. Congress Entomol./symp. on the fruit fly problems, Naha, Japan, Kyoto. Google Scholar

3.

A. P. Economopoulos 2002. The olive fruit fly, Bactrocera (Dacus) oleae (Gmelin) (Diptera: Tephritidae): its importance and control; review SIT research and pilot testing. Rept. Int. Atomic Energy Agency (IAEA), Vienna, Austria. 44pp. Google Scholar

4.

W. Enkerlin , and J. Munford 1997. Economic evaluation pf three alternative methods for control of the Mediterranean fruit fly (Diptera: Tephritidae) in Israel, Palestinian territories, and Jordan. J. Econ. Entomol. 90: 1066–1072 pp. Google Scholar

5.

H. Genc 2008. Modified agar based diet for laboratory rearing of olive fruit fly Bactrocera oleae Gmelin (Diptera:Tephritidae), Florida Entomol. 9(4): 651–656. Google Scholar

6.

H. Genc , and J. L. Nation 2008. Maintaining of Bactrocera oleae (Gmelin.) (Diptera:Tephritidae) colony on its natural host in the laboratory. J. Pest Sei., 81(3): 167–174 pp. Google Scholar

7.

K. S. Hagen 1966. Dependence of the olive fly Dacus oleae larvae on symbiosis with Pseudomonas savastanoi for the utilization of olive. Nat (Lond) 209: 423–424. Google Scholar

8.

K. S. Hagen , L. Santas , and A. Tsecouras 1963. A technique of culturing the olive fruit fly, Dacus oleae Gmel. on synthetic media under xenic conditions, pp. 33–356 In Radiation and radioisotopes applied to insects of agricultural importance. Proc. Symp., Athens, 22–26 April 1963, International Atomic Agency, Vienna, STI/PUB/74. Google Scholar

9.

J. Hendrichs , A. S. Robinson , J. P. Cayol , and W. Enkerlin 2002. Medfly areawide sterile insect technique programmes for prevention, suppression or eradication: the importance of mating behavior studies. Florida Entomol. 85: 1–13. Google Scholar

10.

M. Loukas , Economopoulos ., A. P ., E. Zouros , and Y. Vergini 1985. Genetic changes in artificially reared colonies of the olive fruit fly. Ann. Entomol. Soc. Am. 78: 159–65. Google Scholar

11.

T. E. Mittler , and J. A. Tsitsipis 1973. Economical rearing of larvae of the olive fruit fly, Dacus oleae, on a liquid diet offered on cotton toweling. Entomol. Exp. Appl. 16: 292–293. Google Scholar

12.

G. Skavdis , H. Genc , and J. Vontas 2008. Detection of iAChE organophosphate resistance in Bactrocera oleae from Turkey, XXIII Int. Congress of Entomol., 6–12 Temmuz, Durban, South Africa. 7 pp. Google Scholar

13.

G.M. Silva 1970. Sterile-male technique for control of the olive fly. Review of work on rearing and radiation, pp. 119–130 In Sterile-male technique for control of the fruit flies. International Atomic Energy Agency, Vienna, STI/PUB/276. Google Scholar

14.

J. A. Tsitsipis , and A. Kontos 1983. Improved solid adult diet for the olive fruit fly Dacus oleae. Entomol. Hellenica 1: 24–29. Google Scholar

15.

M. E. Tzanakakis 1971. Rearing methods for the olive fruit fly Dacus oleae (Gmelin). Ann. School of Agric. For., Univ. Thessaloniki 14: 293–326. Google Scholar

16.

J. G. Vontas , J. Hejazi , N. Hawkes , N. Cosmidis ., M. Loukas ., and J. Hemingway 2002. Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase in the olive fruit fly Bactrocera oleae. Insect Mol. Biol. 11: 329–336 pp. Google Scholar

17.

H. V. Weens , and J. L. Nation . 2003. Olive Fruit Fly, Bactrocera oleae (Gmelin) (Insecta:Diptera:Tephritidae). University of Florida Extension, IFAS, EENY-113 document (originally published as DPI Entomology Circular no.44). Google Scholar
Hanıfe Genç "Possible Residual Effect of Pesticide on Bactrocera oleae Rossı (Dıptera:Tephrıtıdae) Adults," Florida Entomologist 95(3), 777-778, (1 September 2012). https://doi.org/10.1653/024.095.0332
Published: 1 September 2012
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