Two new species of treelets/shrubs of the family Rutaceae, known only from the remnant wet forests of Province Los Ríos in western Ecuador, are described and illustrated. Amyris centinelensis is characterized by the unifoliolate large leaves, long petioles, and large fruits, the largest among the South American unifoliolate species. Zanthoxylum bonifaziae is distinguished by the large paripinnate leaves, absence of spines, and mainly by the distinctive short, cauline inflorescences. Their relationships with closely related species are discussed.