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1 January 2000 THE EFFECT OF RETINOIC ACID ON THE PROPORTION OF INSULIN CELLS IN THE DEVELOPING CHICK PANCREAS
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Abstract

We assessed the potential role of all-trans-retinoic acid on the developing chick pancreas, specifically with regard to the proportions of insulin cells. The endodermal component of the dorsal pancreatic bud of 5-d-old chick embryos was cultured on Matrigel. Retinoic acid (10−6 or 10−5 M) was added to a standard serum-free medium, Ham's F12 containing insulin, transferrin and selenium (F12.ITS). Control grafts were cultured in F12.ITS alone or in F12.ITS with DMSO (the diluent for retinoic acid). After 7 d the explants were retrieved, freeze-dried, vapor-fixed, and embedded in resin. Endocrine cell types were identified by immunocytochemistry. The numbers of insulin cells were expressed as a proportion of the sum of insulin plus glucagon cells. Retinoic acid had a dose-related effect; the proportion of insulin cells in explants treated with the lower dose of retinoic acid (10−6 M) was more than twice the proportion of insulin cells in explants treated with the higher dose (10−5 M) of retinoic acid and more than three times that of the control grafts.

CLEM PENNY and BEVERLEY KRAMER "THE EFFECT OF RETINOIC ACID ON THE PROPORTION OF INSULIN CELLS IN THE DEVELOPING CHICK PANCREAS," In Vitro Cellular & Developmental Biology - Animal 36(1), 14-18, (1 January 2000). https://doi.org/10.1290/1071-2690(2000)036<0014:TEORAO>2.0.CO;2
Received: 17 March 1999; Accepted: 1 July 1999; Published: 1 January 2000
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