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1 February 2000 ADAPTATION OF AN INSECT CELL LINE OF SPODOPTERA FRUGIPERDA TO GROW AT 37° C: CHARACTERIZATION OF AN ENDODIPLOID CLONE
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Abstract

Sf21 and Sf9 cell lines established from the lepidoptera Spodoptera frugiperda do not display major induction of heat shock proteins when exposed to a temperature of 37° C. After some months of adaptation at 37° C we obtained two new cell lines, Sf21-HT and Sf9-HT, which have now been established for several years in our laboratory. The Sf9-HT line displays a slightly shorter doubling time at 37° C than the wild type at 28° C, but cell lethality gives rise to an earlier growth arrest. The composition of total lipid extract from heat-adapted cells reveals a higher sphingomyelin to phosphatidylcholine ratio and a higher percentage of saturated fatty acids, which are expected for the lower membrane fluidity, required for thermotolerance. The cell volume of Sf9-HT is doubled, and by flow cytometry we showed that the DNA content is twice that in the parental cell line. Karyotypic examination of metaphasic cells achieved under epifluorescence microscopy revealed a doubled chromosome number in Sf9-HT.

MURIEL GERBAL, PHILIPPE FOURNIER, PATRICK BARRY, MARCEL MARILLER, FRANÇOISE ODIER, GÉRARD DEVAUCHELLE, and MARTINE DUONOR-CERUTTI "ADAPTATION OF AN INSECT CELL LINE OF SPODOPTERA FRUGIPERDA TO GROW AT 37° C: CHARACTERIZATION OF AN ENDODIPLOID CLONE," In Vitro Cellular & Developmental Biology - Animal 36(2), 117-124, (1 February 2000). https://doi.org/10.1290/1071-2690(2000)036<0117:AOAICL>2.0.CO;2
Received: 8 March 1999; Accepted: 1 August 1999; Published: 1 February 2000
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