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1 March 2000 CORTICOTROPIN RELEASING HORMONE AND RELATED PEPTIDES CAN ACT AS BIOREGULATORY FACTORS IN HUMAN KERATINOCYTES
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Abstract

Following previous findings in human skin of the functional expression of genes for the corticotropin releasing hormone (CRH) receptor type 1 (CRH-R1) and CRH itself, we searched for local phenotypic effects for peptides related to CRH. We now report that CRH, sauvagine, and urocortin inhibit proliferation of human HaCaT keratinocytes in a dose-dependent manner. The peptides produced variable cyclic adenosine 3′:5′-monophosphate stimulation, with CRH having the highest potency. Binding of iodine 125 CRH to intact keratinocytes was inhibited by increasing doses of CRH, sauvagine, or urocortin, all showing equal inhibitory potency. Immunocytochemistry identified CRH-R1 immunoreactivity in HaCaT keratinocytes. In conclusion, CRH (exogenous or produced locally) and the related urocortin and sauvagine peptides can modify human keratinocyte phenotype through a receptor-mediated pathway.

ANDRZEJ T. SLOMINSKI, BIRGIT ROLOFF, BLAZEJ ZBYTEK, EDWARD T. WEI, KLAUS FECHNER, JONATHAN CURRY, and JACOBO WORTSMAN "CORTICOTROPIN RELEASING HORMONE AND RELATED PEPTIDES CAN ACT AS BIOREGULATORY FACTORS IN HUMAN KERATINOCYTES," In Vitro Cellular & Developmental Biology - Animal 36(3), 211-216, (1 March 2000). https://doi.org/10.1290/1071-2690(2000)036<0211:CRHARP>2.0.CO;2
Received: 9 August 1999; Accepted: 1 October 1999; Published: 1 March 2000
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