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1 June 2000 CULTURE IN THE ROTATING-WALL VESSEL AFFECTS RECOMBINANT PROTEIN PRODUCTION CAPABILITY OF TWO INSECT CELL LINES IN DIFFERENT MANNERS
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Abstract

The production of recombinant secreted alkaline phosphatase protein in virally infected insect cells was studied in shaker flask and high aspect rotating-wall vessel (HARV) culture. Two commonly used cell lines, Spodoptera frugiperda Sf-9 (Sf-9) and a nonaggregating isolate of the Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) cell line, Trichoplusia ni Tn-5B1-4-NA (Tn-5B1-4-NA), were used and monitored for 120-h postinfection. Different responses to culture in the HARV were seen in the two cell lines. While the Sf-9 cell line was able to produce slightly greater amounts of recombinant protein in the HARV than in shaker flask controls, the Tn-5B1-4-NA cell line produced significantly lesser amounts in the HARV than in the shaker flasks. Both cell lines exhibited longer life spans and longer periods of protein production in HARV culture than in shaker flask culture, presumably due to lower levels of shear encountered in the HARV. The important difference was in the protein production rate responses of the two cell lines. While the protein production rates of Sf-9 cells were comparable in both HARV and shaker flask cultures, the protein production rates of Tn-5B1-4-NA cells were much lower in HARV culture than in shaker flask cultures. The conclusion is drawn that cell line–specific adaptation to the HARV strongly influences recombinant protein production.

MARK A. SAARINEN and DAVID W. MURHAMMER "CULTURE IN THE ROTATING-WALL VESSEL AFFECTS RECOMBINANT PROTEIN PRODUCTION CAPABILITY OF TWO INSECT CELL LINES IN DIFFERENT MANNERS," In Vitro Cellular & Developmental Biology - Animal 36(6), 362-366, (1 June 2000). https://doi.org/10.1290/1071-2690(2000)036<0362:CITRWV>2.0.CO;2
Received: 2 October 1999; Accepted: 1 February 2000; Published: 1 June 2000
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