Translator Disclaimer
1 July 2003 FORTIFICATION OF A PROTEIN-FREE CELL CULTURE MEDIUM WITH PLANT PEPTONES IMPROVES CULTIVATION AND PRODUCTIVITY OF AN INTERFERON-γ–PRODUCING CHO CELL LINE
Author Affiliations +
Abstract

A strong tendency is currently emerging to remove not only serum but also any product of animal origin from animal cell culture media during production of recombinant proteins. This should facilitate downstream processing and improve biosafety. One way consists in the fortification of protein-free nutritive media with plant protein hydrolysates. To investigate the effects of plant peptones on mammalian cell cultivation and productivity, CHO 320 cells, a clone of CHO K1 cells genetically modified to secrete human interferon-γ (IFN-γ), were first adapted to cultivation in suspension in a protein-free medium. Both cell growth and IFN-γ secretion were found to be equivalent to those reached in serum-containing medium. Eight plant peptones, selected on the basis of their content in free amino acids and oligopeptides, as well as molecular weight distribution of oligopeptides, were tested for their ability to improve culture parameters. These were improved in the presence of three peptones, all having an important fraction of oligopeptides ranging from 1 to 10 kDa and a small proportion of peptides higher than 10 kDa. These peptones do not seem to add significantly to the nutritive potential to basal protein-free nutritive medium. Nevertheless, supplementation of an oligopeptide-enriched wheat peptone improved cell growth by up to 30% and IFN-γ production by up to 60% in shake-flask experiments. These results suggest that the use of plant peptones with potential growth factor–like or antiapoptotic bioactivities could improve mammalian cell cultivation in protein-free media while increasing the product biosafety.

CAROLINE C. BURTEAU, FRANÇIS R. VERHOEYE, JOHANN F. MOLS, JEAN-SÉBASTIEN BALLEZ, SPIROS N. AGATHOS, and YVES-JACQUES SCHNEIDER "FORTIFICATION OF A PROTEIN-FREE CELL CULTURE MEDIUM WITH PLANT PEPTONES IMPROVES CULTIVATION AND PRODUCTIVITY OF AN INTERFERON-γ–PRODUCING CHO CELL LINE," In Vitro Cellular & Developmental Biology - Animal 39(7), 291-296, (1 July 2003). https://doi.org/10.1290/1543-706X(2003)039<0291:FOAPCC>2.0.CO;2
Received: 10 June 2003; Accepted: 1 August 2003; Published: 1 July 2003
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top