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1 September 2003 ESTROGENIC COMPOUNDS SUPPRESSED INTERFERON-GAMMA PRODUCTION IN MOUSE SPLENOCYTES THROUGH DIRECT CELL–CELL INTERACTION
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Abstract
Here, we reported the effects of 17β-estradiol (E2), isoflavone genistein (Gen), and daidzein (Dai) on the production of interferon (IFN)-γ by splenocytes isolated from C57BL/6N mice. When mouse splenocytes were stimulated with lipopolysaccharide, E2, Gen, and Dai suppressed the production of IFN-γ. However, when only nonadherent cell populations of splenocytes were tested, none of these estrogenic compounds suppressed IFN-γ production. This result indicates that IFN-γ production by nonadherent cell populations of splenocytes treated with estrogens is regulated by adherent cell populations. Moreover, direct cell–cell interaction between both populations was necessary for suppression of IFN-γ production by nonadherent populations. In addition, E2 conjugated with bovine serum albumin inhibited IFN-γ production as well as E2. This result suggests that the plasma membrane–associated estrogen receptor plays a prominent role in this suppression mechanism.
MAKO NAKAYA, MASAO YAMASAKI, YOSHIYUKI MIYAZAKI, HIROFUMI TACHIBANA and KOJI YAMADA "ESTROGENIC COMPOUNDS SUPPRESSED INTERFERON-GAMMA PRODUCTION IN MOUSE SPLENOCYTES THROUGH DIRECT CELL–CELL INTERACTION," In Vitro Cellular & Developmental Biology - Animal 39(8), (1 September 2003). https://doi.org/10.1290/1543-706X(2003)039<0383:ECSIPI>2.0.CO;2
Received: 9 September 2003; Accepted: 4 November 2003; Published: 1 September 2003
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