Translator Disclaimer
1 September 2003 ANTAGONISTIC EFFECTS OF SIMULTANEOUS EXPOSURE OF ERGOT ALKALOIDS ON KIDNEY ADENOSINE TRIPHOSPHATASE SYSTEM
Author Affiliations +
Abstract

Much of the research on fescue toxicosis has concentrated on evaluating animal response to grazing endophyte-infected (E ) versus endophyte-free tall fescue or the effects of single toxins such as ergonovine (EN), ergovaline (EV), or ergotamine (ET) on animal performance. Such approaches have eliminated the opportunity to test the possible additive, synergistic, or antagonistic interactions of one or more ergot alkaloids with the other ergot alkaloids found in E tall fescue. This study was conducted to determine the effects of simultaneous exposure of pairs of EN, EV, and ET on the kidney adenosine triphosphatase (ATPase) system in vitro. Tests were performed using three separate rat kidney homogenates and were repeated four times at concentrations of 0, 75, and 200 μM. Individually, EN, EV, and ET induced dose-dependent inhibitions of kidney Na /K ATPase, with EN being most potent, followed by purified EV, and then by ET. The ergot alkaloids inhibited Mg2 ATPase to a lesser degree than Na /K ATPase, with EN again being the most potent toxin. Simultaneous exposure to any combination of the ergot alkaloid pairs tested (EV ET, EV EN, and ET EN) resulted in significant interactions (P < 0.05), indicating antagonistic effects on the inhibition of Na /K ATPase and Mg2 ATPase for most concentration combinations. These interactions suggest that in studies of the effects of any ergot alkaloid on animal performance, effects of other ergot alkaloids may also be present. Effects may not be additive, as was the case in this study, and the presence of one toxin may enhance or hinder the effectiveness of others.

ALI S. MOUBARAK, ZELPHA B. JOHNSON, and CHARLES F. ROSENKRANS Jr. "ANTAGONISTIC EFFECTS OF SIMULTANEOUS EXPOSURE OF ERGOT ALKALOIDS ON KIDNEY ADENOSINE TRIPHOSPHATASE SYSTEM," In Vitro Cellular & Developmental Biology - Animal 39(8), 395-398, (1 September 2003). https://doi.org/10.1290/1543-706X(2003)039<0395:AEOSEO>2.0.CO;2
Received: 7 August 2003; Accepted: 3 December 2003; Published: 1 September 2003
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top